MelampsoraV2, France, Analysis, bibRecord, 000149

RNA-Seq of Early-Infected Poplar Leaves by the Rust Pathogen Melampsora larici-populina Uncovers PtSultr3;5, a Fungal-Induced Host Sulfate Transporter

Identifieur interne : 000149 ( France/Analysis ); précédent : 000148; suivant : 000150

RNA-Seq of Early-Infected Poplar Leaves by the Rust Pathogen Melampsora larici-populina Uncovers PtSultr3;5, a Fungal-Induced Host Sulfate Transporter

Auteurs : Benjamin Petre [France] ; Emmanuelle Morin [France] ; Emilie Tisserant [France] ; Stéphane Hacquard [France] ; Corinne Da Silva [France] ; Julie Poulain [France] ; Christine Delaruelle [France] ; Francis Martin [France] ; Nicolas Rouhier [France] ; Annegret Kohler [France] ; Sébastien Duplessis [France]

Source :

PLoS ONE [ 1932-6203 ] ; 2012.

RBID : PMC:3431362

Descripteurs français

English descriptors

KwdEn :
- Basidiomycota (physiology), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (genetics), Host-Pathogen Interactions (genetics), Membrane Transport Proteins (genetics), Membrane Transport Proteins (metabolism), Oligonucleotide Array Sequence Analysis (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (genetics), Plant Leaves (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (microbiology), RNA, Messenger (genetics), RNA, Messenger (metabolism), RNA, Plant (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sulfates (metabolism), Transcriptome (genetics).
MESH :
- chemical , genetics : Membrane Transport Proteins, Plant Proteins, RNA, Messenger, RNA, Plant.
- genetics : Genes, Plant, Host-Pathogen Interactions, Plant Diseases, Plant Leaves, Populus, Transcriptome.
- chemical , metabolism : Membrane Transport Proteins, Plant Proteins, RNA, Messenger, Sulfates.
- microbiology : Plant Diseases, Plant Leaves, Populus.
- physiology : Basidiomycota.
- Gene Expression Profiling, Gene Expression Regulation, Plant, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

Biotroph pathogens establish intimate interactions with their hosts that are conditioned by the successful secretion of effectors in infected tissues and subsequent manipulation of host physiology. The identification of early-expressed pathogen effectors and early-modulated host functions is currently a major goal to understand the molecular basis of biotrophy. Here, we report the 454-pyrosequencing transcriptome analysis of early stages of poplar leaf colonization by the rust fungus Melampsora larici-populina. Among the 841,301 reads considered for analysis, 616,879 and 649 were successfully mapped to Populus trichocarpa and M. larici-populina genome sequences, respectively. From a methodological aspect, these results indicate that this single approach is not appropriate to saturate poplar transcriptome and to follow transcript accumulation of the pathogen. We identified 19 pathogen transcripts encoding early-expressed small-secreted proteins representing candidate effectors of interest for forthcoming studies. Poplar RNA-Seq data were validated by oligoarrays and quantitatively analysed, which revealed a highly stable transcriptome with a single transcript encoding a sulfate transporter (herein named PtSultr3;5, POPTR_0006s16150) showing a dramatic increase upon colonization by either virulent or avirulent M. larici-populina strains. Perspectives connecting host sulfate transport and biotrophic lifestyle are discussed.

Url:

DOI: 10.1371/journal.pone.0044408
PubMed: 22952974
PubMed Central: 3431362

Affiliations:

Links to Exploration step

PMC:3431362

Le document en format XML

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 Uncovers <italic>PtSultr3;5</italic>
, a Fungal-Induced Host Sulfate Transporter</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">RNA-Seq of Early-Infected Poplar Leaves by the Rust Pathogen <italic>Melampsora larici-populina</italic>
 Uncovers <italic>PtSultr3;5</italic>
, a Fungal-Induced Host Sulfate Transporter</title>
<author><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name>
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</author>
<author><name sortKey="Kohler, Annegret" sort="Kohler, Annegret" uniqKey="Kohler A" first="Annegret" last="Kohler">Annegret Kohler</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Unité Mixte de Recherche 1136 ‘Interactions Arbres/Microorganismes’, INRA (Institut National de la Recherche Agronomique)/Université de Lorraine, Centre INRA de Nancy, Champenoux, France</addr-line>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Unité Mixte de Recherche 1136 ‘Interactions Arbres/Microorganismes’, INRA (Institut National de la Recherche Agronomique)/Université de Lorraine, Centre INRA de Nancy, Champenoux</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Champenoux</settlement>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name>
<affiliation wicri:level="4"><nlm:aff id="aff1"><addr-line>Unité Mixte de Recherche 1136 ‘Interactions Arbres/Microorganismes’, INRA (Institut National de la Recherche Agronomique)/Université de Lorraine, Centre INRA de Nancy, Champenoux, France</addr-line>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Unité Mixte de Recherche 1136 ‘Interactions Arbres/Microorganismes’, INRA (Institut National de la Recherche Agronomique)/Université de Lorraine, Centre INRA de Nancy, Champenoux</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Champenoux</settlement>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (physiology)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (genetics)</term>
<term>Host-Pathogen Interactions (genetics)</term>
<term>Membrane Transport Proteins (genetics)</term>
<term>Membrane Transport Proteins (metabolism)</term>
<term>Oligonucleotide Array Sequence Analysis (MeSH)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (microbiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (microbiology)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA, Plant (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Sulfates (metabolism)</term>
<term>Transcriptome (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term>
<term>ARN messager (génétique)</term>
<term>ARN messager (métabolisme)</term>
<term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Basidiomycota (physiologie)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Gènes de plante (génétique)</term>
<term>Interactions hôte-pathogène (génétique)</term>
<term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Populus (génétique)</term>
<term>Populus (microbiologie)</term>
<term>Protéines de transport membranaire (génétique)</term>
<term>Protéines de transport membranaire (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>RT-PCR (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Sulfates (métabolisme)</term>
<term>Séquençage par oligonucléotides en batterie (MeSH)</term>
<term>Transcriptome (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Transport Proteins</term>
<term>Plant Proteins</term>
<term>RNA, Messenger</term>
<term>RNA, Plant</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Plant</term>
<term>Host-Pathogen Interactions</term>
<term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Populus</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term>
<term>ARN messager</term>
<term>Feuilles de plante</term>
<term>Gènes de plante</term>
<term>Interactions hôte-pathogène</term>
<term>Maladies des plantes</term>
<term>Populus</term>
<term>Protéines de transport membranaire</term>
<term>Protéines végétales</term>
<term>Transcriptome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Transport Proteins</term>
<term>Plant Proteins</term>
<term>RNA, Messenger</term>
<term>Sulfates</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term>
<term>Protéines de transport membranaire</term>
<term>Protéines végétales</term>
<term>Sulfates</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Plant</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>RT-PCR</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Séquençage par oligonucléotides en batterie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Biotroph pathogens establish intimate interactions with their hosts that are conditioned by the successful secretion of effectors in infected tissues and subsequent manipulation of host physiology. The identification of early-expressed pathogen effectors and early-modulated host functions is currently a major goal to understand the molecular basis of biotrophy. Here, we report the 454-pyrosequencing transcriptome analysis of early stages of poplar leaf colonization by the rust fungus <italic>Melampsora larici-populina</italic>
. Among the 841,301 reads considered for analysis, 616,879 and 649 were successfully mapped to <italic>Populus trichocarpa</italic>
 and <italic>M. larici-populina</italic>
 genome sequences, respectively<bold>.</bold>
 From a methodological aspect, these results indicate that this single approach is not appropriate to saturate poplar transcriptome and to follow transcript accumulation of the pathogen. We identified 19 pathogen transcripts encoding early-expressed small-secreted proteins representing candidate effectors of interest for forthcoming studies. Poplar RNA-Seq data were validated by oligoarrays and quantitatively analysed, which revealed a highly stable transcriptome with a single transcript encoding a sulfate transporter (herein named PtSultr3;5, POPTR_0006s16150) showing a dramatic increase upon colonization by either virulent or avirulent <italic>M. larici-populina</italic>
 strains. Perspectives connecting host sulfate transport and biotrophic lifestyle are discussed.</p>
</div>
</front>
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<author><name sortKey="Braun, E" uniqKey="Braun E">E Braun</name>
</author>
<author><name sortKey="Nettleton, D" uniqKey="Nettleton D">D Nettleton</name>
</author>
</analytic>
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</author>
<author><name sortKey="Major, I" uniqKey="Major I">I Major</name>
</author>
<author><name sortKey="Rouhier, N" uniqKey="Rouhier N">N Rouhier</name>
</author>
<author><name sortKey="Duplessis, S" uniqKey="Duplessis S">S Duplessis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kataoka, T" uniqKey="Kataoka T">T Kataoka</name>
</author>
<author><name sortKey="Hayashi, N" uniqKey="Hayashi N">N Hayashi</name>
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</author>
<author><name sortKey="Takahashi, H" uniqKey="Takahashi H">H Takahashi</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wienkoop, S" uniqKey="Wienkoop S">S Wienkoop</name>
</author>
<author><name sortKey="Saalbach, G" uniqKey="Saalbach G">G Saalbach</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krusell, L" uniqKey="Krusell L">L Krusell</name>
</author>
<author><name sortKey="Krause, K" uniqKey="Krause K">K Krause</name>
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<author><name sortKey="Ott, T" uniqKey="Ott T">T Ott</name>
</author>
<author><name sortKey="Desbrosses, G" uniqKey="Desbrosses G">G Desbrosses</name>
</author>
<author><name sortKey="Kramer, U" uniqKey="Kramer U">U Kramer</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Felten, J" uniqKey="Felten J">J Felten</name>
</author>
<author><name sortKey="Kohler, A" uniqKey="Kohler A">A Kohler</name>
</author>
<author><name sortKey="Morin, E" uniqKey="Morin E">E Morin</name>
</author>
<author><name sortKey="Bhalerao, Rp" uniqKey="Bhalerao R">RP Bhalerao</name>
</author>
<author><name sortKey="Palme, K" uniqKey="Palme K">K Palme</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mukhtar, Ms" uniqKey="Mukhtar M">MS Mukhtar</name>
</author>
<author><name sortKey="Carvunis, A R" uniqKey="Carvunis A">A-R Carvunis</name>
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<author><name sortKey="Dreze, M" uniqKey="Dreze M">M Dreze</name>
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<author><name sortKey="Steinbrenner, J" uniqKey="Steinbrenner J">J Steinbrenner</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baxter, L" uniqKey="Baxter L">L Baxter</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Spanu, Pd" uniqKey="Spanu P">PD Spanu</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Antony, G" uniqKey="Antony G">G Antony</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boch, J" uniqKey="Boch J">J Boch</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, L Q" uniqKey="Chen L">L-Q Chen</name>
</author>
<author><name sortKey="Hou, B H" uniqKey="Hou B">B-H Hou</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Talbot, Nj" uniqKey="Talbot N">NJ Talbot</name>
</author>
</analytic>
</biblStruct>
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</author>
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<author><name sortKey="Smoker, M" uniqKey="Smoker M">M Smoker</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dowkiw, A" uniqKey="Dowkiw A">A Dowkiw</name>
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<author><name sortKey="Husson, C" uniqKey="Husson C">C Husson</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boyle, B" uniqKey="Boyle B">B Boyle</name>
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</author>
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</analytic>
</biblStruct>
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<author><name sortKey="Levee, V" uniqKey="Levee V">V Levée</name>
</author>
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</author>
<author><name sortKey="Seguin, A" uniqKey="Seguin A">A Séguin</name>
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</analytic>
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</author>
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<author><name sortKey="Pfisterer, T" uniqKey="Pfisterer T">T Pfisterer</name>
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<author><name sortKey="Muller, Weg" uniqKey="Muller W">WEG Muller</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gremme, G" uniqKey="Gremme G">G Gremme</name>
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<author><name sortKey="Brendel, V" uniqKey="Brendel V">V Brendel</name>
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<author><name sortKey="Kurtz, S" uniqKey="Kurtz S">S Kurtz</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Milne, I" uniqKey="Milne I">I Milne</name>
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<author><name sortKey="Bayer, M" uniqKey="Bayer M">M Bayer</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ruepp, A" uniqKey="Ruepp A">A Ruepp</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marioni, Jc" uniqKey="Marioni J">JC Marioni</name>
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<biblStruct><analytic><author><name sortKey="Tsai, C J" uniqKey="Tsai C">C-J Tsai</name>
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</biblStruct>
<biblStruct><analytic><author><name sortKey="Hamberger, B" uniqKey="Hamberger B">B Hamberger</name>
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</biblStruct>
<biblStruct><analytic><author><name sortKey="Miranda, M" uniqKey="Miranda M">M Miranda</name>
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<biblStruct><analytic><author><name sortKey="Azaiez, A" uniqKey="Azaiez A">A Azaiez</name>
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<author><name sortKey="Boyle, B" uniqKey="Boyle B">B Boyle</name>
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<author><name sortKey="Levee, V" uniqKey="Levee V">V Levée</name>
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<author><name sortKey="Seguin, A" uniqKey="Seguin A">A Séguin</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Grand Est</li>
<li>Lorraine (région)</li>
<li>Île-de-France</li>
</region>
<settlement><li>Champenoux</li>
<li>Évry (Essonne)</li>
</settlement>
<orgName><li>Université de Lorraine</li>
</orgName>
</list>
<tree><country name="France"><region name="Grand Est"><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name>
</region>
<name sortKey="Da Silva, Corinne" sort="Da Silva, Corinne" uniqKey="Da Silva C" first="Corinne" last="Da Silva">Corinne Da Silva</name>
<name sortKey="Delaruelle, Christine" sort="Delaruelle, Christine" uniqKey="Delaruelle C" first="Christine" last="Delaruelle">Christine Delaruelle</name>
<name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name>
<name sortKey="Hacquard, Stephane" sort="Hacquard, Stephane" uniqKey="Hacquard S" first="Stéphane" last="Hacquard">Stéphane Hacquard</name>
<name sortKey="Kohler, Annegret" sort="Kohler, Annegret" uniqKey="Kohler A" first="Annegret" last="Kohler">Annegret Kohler</name>
<name sortKey="Martin, Francis" sort="Martin, Francis" uniqKey="Martin F" first="Francis" last="Martin">Francis Martin</name>
<name sortKey="Morin, Emmanuelle" sort="Morin, Emmanuelle" uniqKey="Morin E" first="Emmanuelle" last="Morin">Emmanuelle Morin</name>
<name sortKey="Poulain, Julie" sort="Poulain, Julie" uniqKey="Poulain J" first="Julie" last="Poulain">Julie Poulain</name>
<name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name>
<name sortKey="Tisserant, Emilie" sort="Tisserant, Emilie" uniqKey="Tisserant E" first="Emilie" last="Tisserant">Emilie Tisserant</name>
</country>
</tree>
</affiliations>
</record>

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   |wiki=    Bois
   |area=    MelampsoraV2
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     PMC:3431362
   |texte=   RNA-Seq of Early-Infected Poplar Leaves by the Rust Pathogen Melampsora larici-populina Uncovers PtSultr3;5, a Fungal-Induced Host Sulfate Transporter
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Serveur d'exploration Melampsora (ISTEX)

RNA-Seq of Early-Infected Poplar Leaves by the Rust Pathogen Melampsora larici-populina Uncovers PtSultr3;5, a Fungal-Induced Host Sulfate Transporter

RNA-Seq of Early-Infected Poplar Leaves by the Rust Pathogen Melampsora larici-populina Uncovers PtSultr3;5, a Fungal-Induced Host Sulfate Transporter

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